Mice are evaluated daily using a clinical scoring system for 25-50 days. Mice develop a "classic" self-limited monophasic EAE with ascending flaccid paralysis within 9-14 days after immunization. The immunogenic epitope MOG35-55 is suspended in complete Freund's adjuvant (CFA) prior to immunization and pertussis toxin is applied on the day of immunization and two days later. C57BL/6 mice are the commonly used strain for transgenic mouse construction and respond among others to myelin oligodendrocyte glycoprotein (MOG). EAE susceptibility and phenotype depends on the chosen antigen and rodent strain. Due to the low immunogenic potential of these peptides, strong adjuvants are used. Therefore, mice are immunized with CNS homogenates or peptides of myelin proteins. It is especially suited for investigating the effects of drugs or of particular genes by using transgenic mice challenged by autoimmune neuroinflammation. Actively-induced EAE in mice is the easiest inducible model with robust and replicable results. There is a broad diversity of EAE models which reflect different clinical, immunological and histological aspects of human MS. Experimental autoimmune encephalomyelitis (EAE) is the most common animal model for MS sharing many clinical and pathophysiological features. MS therapy is only partially effective so far and research efforts continue to expand our knowledge on the pathophysiology of the disease and to develop novel treatment strategies. While the exact etiology of the disease is yet unclear, autoreactive T lymphocytes are thought to play a central role in its pathophysiology. C57BL/6 mice were submitted to experimental autoimmune encephalomyelitis (EAE) induction and treated with a MOG + PARI association by epicutaneous route at days 3 and 11 after disease induction. Considering the tolerogenic effects of active vitamin D. Multiple sclerosis is a chronic neuroinflammatory demyelinating disorder of the central nervous system with a strong neurodegenerative component. Experimental autoimmune encephalomyelitis (EAE) is an animal model to study multiple sclerosis (MS).
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